Ultrafast synthesis of isoquercitrin by enzymatic hydrolysis of rutin in a continuous-flow microreactor

作者：Wang, Jun; Gong, An; Gu, Shuangshuang; Cui, Hongsheng; Wu, Xiangyang^*

来源：Journal of the Serbian Chemical Society, 2015, 80(7): 853-866.

DOI：10.2298/JSC150914112W

• biocatalysis
• continuous flow
• hesperidinase
• isoquercitrin
• microreactor

Isoquercitrin is a rare flavonol glycoside with a wide range of biological activities and is a key synthetic intermediate for the. production of enzymatically modified isoquercitrin. In order to establish an ultrafast bioprocess for obtaining isoquercitrin, a novel continuous flow biosynthesis of isoquercitrin using the hesperidinase-catalyzed hydrolysis of rutin in a glass polydimethylsiloxane (PDMS) microreactor was first performed. Using the developed microchannel reactor (200 mu m width, 50 mu m depth and 2 m length) with one T-shaped inlet and one outlet, the maximum yield of isoquercitrin (98.6 %) was achieved in a short time (40 min) under the following optimum conditions: rutin concentration at 1 g L-1, hesperidinase concentration at 0.1 g mL(-1), reaction temperature 40 degrees C, and a flow rate of 2 mu L min(-1). The value of the activation energy, E-a, of the enzymatic reaction was 4.61 kJ mol(-1), and the teaction rate and volume productivity were approximately 16.1-fold and 30 % higher, respectively, than those in a batch reactor were. Thus, the use of a continuous-flow microreactor for the enzymatic hydrolysis of rutin is an efficient and simple approach to achieve a relatively high yield of isoquercitrin.

• 出版日期2015
• 单位江苏大学; 江苏科技大学