Automated determination of Rifamycins making use of MPA-CdTe quantum dots

作者：Jimenez Lopez J; Molina Garcia L; Rodrigues S S M; Santos J L M; Ortega Barrales P; Ruiz Medina A

来源：Journal of Luminescence, 2016, 175: 158-164.

DOI：10.1016/j.jlumin.2016.02.036

• Rifamycins
• Clinical analysis
• Luminescence
• CdTe quantum dots

Rifamycins are a group of antibiotics particularly effective against mycobacteria and they are used for the treatment of important diseases and disorders such as tuberculosis, cancer, hepatic encephalopathy or intestinal infections. Taking into account the great clinical potential of these drugs it is important to develop a rapid, simple and reliable strategy for its quality control. This paper presents an automated quantum dots-based analytical method making use of a multicommutated flow system and the quenching effect that rifampicin and rifaximin, two important Rifamycin derivatives, have on the fluorescence of water-soluble mercaptopropionic acid-capped CdTe quantum dots.
Under the optimized conditions, the relationship between the fluorescence intensity of the quantum dots and rifampicin or rifaximin concentrations were linear in the range of 5-80 and 3-40 mu g mL(-1), with a detection limit of 1.5 and 1.0 mu g mL(-1), respectively. Relative standard deviations (RSD) lower than 3% were observed in all cases. A sample throughput of 70 determinations per hour and good recoveries were also achieved. The proposed method was satisfactorily applied to the determination of rifamycins in pharmaceutical formulations and human urine.

• 出版日期2016-7