Comparative analysis of micro-RNAs in human papillomavirus-positive versus -negative oropharyngeal cancers

作者：Mirghani Haitham^*; Ugolin Nicolas; Ory Catherine; Goislard Maud; Lefevre Marine; Baulande Sylvain; Hofman Paul; St Guily Jean Lacau; Chevillard Sylvie; Lacave Roger

来源：Head and Neck-Journal for the Sciences and Specialties of the Head and Neck, 2016, 38(11): 1634-1642.

DOI：10.1002/hed.24487

• oropharyngeal/oropharynx cancer
• human papillomavirus 16
• microarray
• micro/miRNA
• gene expression profile
• PI3K pathway
• Wnt pathway

Background. Oncogenic mechanisms of human papillomavirus (HPV)-positive oropharyngeal cancer are still poorly characterized. Analysis of their microRNA expression profile might provide valuable information.
Methods. The microRNA expression profiles were analyzed by microarrays in 26 oropharyngeal cancers. A microRNA signature specific to HPV-status was identified by analyzing a learning/training set consisting of 16 oropharyngeal cancers. The robustness of this signature was further confirmed by blind case-by-case classification of a validation set composed of 10 independent tumors. Putative targeted molecular pathways were proposed using DIANA miRPath online software (http://microrna.gr/mirpath).
Results. We have identified 25 miRNA signatures, which discriminates HPV16-positive oropharyngeal cancer from their HPV-negative counterparts. These 25 microRNAs play a potential role in Wnt and PI3K-pathways, cell-adhesion/cell-polarity, and the cytoskeleton regulation.
Conclusion. Our study contributes to a better understanding of pathogenic mechanisms involved in the development of HPV-positive oropharyngeal cancer and in the identification of potential therapeutic molecular targets.

• 出版日期2016-11
• 单位中国地震局