Aluminum Chloride- and Norepinephrine-Induced Immunotoxicity on Splenic Lymphocytes by Activating beta(2)-AR/cAMP/PKA/NF-kappa B Signal Pathway in Rats

作者：Xiu, Chunyu; Ren, Limin; Li, Miao; Liu, Shiming; Zhu, Yanzhu; Liu, Jianyu; Li, Yanfei^*

来源：Biological Trace Element Research, 2014, 162(1-3): 168-174.

DOI：10.1007/s12011-014-0149-7

• NE
• AlCl3
• Immunotoxicity beta(2)-AR/cAMP/PKA/NF-kappa B pathway
• Rat

We found in our previous research that aluminum (Al) exposure induced immunotoxicity on spleen and increased norepinephrine (NE) content in serum from rats. However, it is unclear how NE is involved in the AlCl3 immunotoxicity on rats. Therefore, this experiment was designed to explore the mechanism of AlCl3 and NE-induced immunotoxicity on the splenic lymphocytes. Eighty male Wistar rats were orally exposed to AlCl3 (0, 64, 128, and 256 mg/kg BW) through drinking water for 120 days. Al contents in brain and spleen; NE contents in serum and in the hypothalamus; beta(2)-AR density; cAMP content; beta(2)-AR, PKA, and NF-kappa B mRNA expression levels; and protein expressions of PKA and nuclear NF-kappa B in splenic lymphocytes of AlCl3-treated rats were examined. The results showed that AlCl3 increased NE content in serum, the beta(2)-AR density, the beta(2)-AR and PKA (C-subunits) mRNA expression levels, cAMP content and the PKA (C-subunits) protein expression levels in lymphocytes, whereas, decreased NE content in the hypothalamus, the NF-kappa B (p65) mRNA expression level and nuclear NF-kappa B (p65) protein expression level in lymphocytes. These results indicated that the accumulated AlCl3 in spleen and the increased NE in serum induced the immunotoxicity on splenic lymphocytes by activating beta(2)-AR/cAMP/PKA/NF-kappa B signal pathway in rats.

• 出版日期2014-12
• 单位东北农业大学; 中国农业科学院