Phenotypic and genotypic antimicrobial resistance of Lactococcus sp, strains isolated from rainbow trout (Oncorhynchus mykiss)

作者：Ture Mustafa^*; Boran Halis

来源：Bulletin of the Veterinary Institute in Pulawy, 2015, 59(1): 37-42.

DOI：10.1515/bvip-2015-0006

• fish
• Lactococeus gurvieae
• antibiotic resistance
• resistance gene
• plasmid

A current profile of antimicrobial resistance and plasmid of 29 Lactococcus garvieae and one Lactococcus lactis strains isolated from rainbow trouts (Oncorhynchus mykiss) from farms throughout Turkey were investigated. All isolates were sensitive to penicillin G (90%), ampicillin (86.7%), florfenicol (83.3%), amoxicillin (80.1%), and tetracycline (73.4%), and resistant to trimethoprim isulfamethoxazole (86.6%) and gentamycin (46.6%) by disc diffusion method. Twenty-eight (93%) isolates had two to seven antibiotic resistance genes (ARGs) determined by PCR. The most prevalent ARGs were tetracycline (tetB), erythromycin (ereB), and beta-lactam (bla(TEM)). Bacterial strains were also screened for plasmid DNA by agarose gel electrophoresis and two strains harboured plasmids, with sizes ranging from 3 to 9 kb.

• 出版日期2015