Molecular simulation of the interaction between novel type rhodanine derivative probe and bovine serum albumin

作者：Yu Jinghua^*; Li Bo; Dai Ping; Ge Shenguang

来源：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy , 2009, 74(1): 277-281.

DOI：10.1016/j.saa.2009.06.013

• Fluorescence spectrum
• Rhodanine derivative
• Bovine serum albumin
• Interaction

The interaction between 3-(4'-methylphenyl)-5-(4'-methyl-2'-sulfophenylazo) rhodanine (M4MRASP) and bovine serum albumin (BSA) was studied by using spectrofluorimetry. It was shown in fluorescence spectrums that the quenching mechanism of BSA by M4MRASP was a static quenching. Meanwhile, the binding constant and binding site numbers were calculated. The action distance (r = 8.03 nm) and energy transfer efficiency (E = 0.12) between donor (BSA) and acceptor (M4MRASP) were obtained according to the theory of Forster non-radiation energy transfer. The effect of M4MRASP on the conformation of BSA was further analyzed by using synchronous fluorescence spectrometry. A new model of the interaction between small organic molecule and biomacromolecule was established. The results offered a reference for the studies on the biological effects and action mechanism of small molecule with protein.

• 出版日期2009-9-15
• 单位济南大学