Antioxidant reactivity toward nitroxide probes anchored into human serum albumin. A new model for studying antioxidant repairing capacity of protein radicals

作者：Aspee Alexis^*; Orrego Alejandra; Alarcon Emilio; Lopez Alarcon Camilo; Poblete Horacio; Gonzalez Nilo Danilo

来源：Bioorganic & Medicinal Chemistry Letters, 2009, 19(22): 6382-6385.

DOI：10.1016/j.bmcl.2009.09.070

• Nitroxide prefluorescent probes
• Antioxidants
• Nitroxide reduction
• Human serum albumin
• Binding constant

A new strategy to evaluate accessibility of antioxidants to radical proteins has been developed using nitroxide prefluorescent probes anchored into human serum albumin (HSA). Binding association constants for the nitroxide probes C(343)T and QT with HSA were 5 x 10(4) and 9 x 10(4) M(-1), respectively. Rate constants for the nitroxide reduction by antioxidants in HSA were determined finding k(HSA)/k(buffer) ratio of 0.8, 1.9, and 0.075 for ascorbic acid, Trolox, and caffeic acid, respectively, for the nitroxide C(343)T reduction.

• 出版日期2009-11-15
• 单位上海生物信息技术研究中心