Acute exposure to waterborne copper inhibits both the excretion and uptake of ammonia in freshwater rainbow trout (Oncorhynchus mykiss)

作者：Lim Michael Yu Ting; Zimmer Alex M^*; Wood Chris M

来源：Comparative Biochemistry and Physiology C-Toxicology & Pharmacology, 2015, 168: 48-54.

DOI：10.1016/j.cbpc.2014.12.002

• Ammonia excretion (J(amm))
• Carbonic anhydrase (CA)
• Copper
• H+-ATPase
• High environmental ammonia (HEA)
• Na+/K+-ATPase
• Rhesus (Rh) glycoproteins
• Sodium uptake (J(in)(Na))

In freshwater fish, exposure to sub-lethal concentrations of waterborne copper (Cu) results in inhibitions of ammonia excretion (J(amm)) and Na+ uptake (rain), yet the mechanisms by which these occur are not fully understood. In the present study, rainbow trout (Oncorhynchus mykiss) fry exposed to 50 mu g/I Cu for 24 h displayed a sustained 40% decrease in J(amm) and a transient 60% decrease in J(in)(Na). Previously, these effects have been attributed to inhibitions of gill Na+/K+-ATPase and/or carbonic anhydrase (CA) activities by Cu. Trout fry did not display significant reductions in the branchial activities of these enzymes or H+-ATPase over 24 h Cu exposure. Recently, Rhesus (Rh) glycoproteins, bi-directional NH3 gas channels, have been implicated in the mechanism of Cu toxicity. Juvenile trout were exposed to nominal 0, 50, and 200 mu g/l Cu for 3-6 h under control conditions (ammonia-free water) followed by 6 h exposure to high environmental ammonia (HEA; 1.5 mmol/l NH4HCO3). HEA led to significant ammonia uptake in control fish (0 mu g/l Cu), and exposure to 50 and 200 mu g/l Cu resulted in significant reductions of ammonia uptake during HEA exposure. This is the first evidence that Cu inhibits both the excretion and uptake of ammonia, implicating bi-directional Rh glycoproteins as a target for Cu toxicity. We propose a model whereby Rh blockade by Cu causes the sustained inhibition of J(amm) and transient inhibition of J(in)(Na), with H+-ATPase potentially aiding in J(in)(Na) recovery. More work is needed to elucidate the role of Rh proteins in sub-lethal Cu toxicity.

• 出版日期2015-2