Adoptive transfer of CD4(+)Foxp3(+) regulatory T cells to C57BL/6J mice during acute infection with Toxoplasma gondii down modulates the exacerbated T(h)1 immune response

作者：Olguin Jonadab E; Fernandez Jacquelina; Salinas Nohemi; Juarez Imelda; Rodriguez Sosa Miriam; Campuzano Jaime; Castellanos Carlos; Saavedra Rafael^*

来源：Microbes and Infection, 2015, 17(8): 586-595.

DOI：10.1016/j.micinf.2015.04.002

• Regulatory T-cells
• Toxoplasma gondii
• Adoptive transfer
• T(h)1 cells
• Interferon-gamma

Infection of C57BL/67 mice with the parasite Toxoplasma gondii triggers a powerful T(h)1 immune response that is detrimental to the host. During acute infection, a reduction in CD4(+)Foxp3(+) regulatory T cells (Treg) has been reported. We studied the role of Treg during T gondii infection by adoptive transfer of cells purified from transgenic Foxp3(EGFP) mice to infected wild type animals. We found a less severe weight loss, a significant delayed mortality in infected Treg-transferred mice, and reduced pathology of the small intestine that were associated with lower IFN-gamma and TNF-alpha levels. Nevertheless, higher cyst number and parasite load in brain were observed in these mice. Treg-transferred infected mice showed reduced levels of both IFN-gamma and TNF-alpha in sera. A reduced number of CD4(+) T cells producing IFN-gamma was detected in these mice, while IL-2 producing CD4(+) T cells were restored to levels nearly similar to uninfected mice. CD25 and CD69 expression of CD4(+) T cells were also down modulated. Our data show that the low Treg cell number are insufficient to modulate the activation of CD4(+) T cells and the production of high levels of IFN-gamma. Thus, a delicate balance between an optimal immune response and its modulation by Treg cells must exist.

• 出版日期2015-8