AFLP genetic diversity analysis in Russian wheat aphid resistant wheat accessions

作者：Srinivas G; Huang Yinghua^*; Carver Brett F; Mornhinweg Do

来源：Euphytica, 2012, 185(1): 27-35.

DOI：10.1007/s10681-011-0605-9

• Wheat
• Resistance
• Biotypes
• Amplified fragment length polymorphism
• Genetic diversity

Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), is an important insect pest which causes severe economic losses in wheat (Triticum spp.). Among the various U.S. RWA biotypes, biotype 1 (RWA1) and biotype 2 (RWA2) are the most prevalent and most virulent on cultivated genotypes. Although many sources of resistance to these biotypes are available among landraces, their relatedness should be characterized to permit their more efficient use in breeding programs. In this study, 38 hexaploid accessions resistant to biotype 1 and/or biotype 2 were evaluated for genetic diversity based on amplified fragment length polymorphisms (AFLP). Fifteen AFLP selective primer combinations were used to genotype these accessions, resulting in 893 amplicons. Of these, 274 (30.6%) informative polymorphic bands were used for genetic diversity analysis. Genetic similarity coefficients ranged from 0.47 to 0.87 among the resistant accessions, indicating high genetic diversity among them. Cluster analysis grouped the 38 accessions into two major clusters, I and II, including resistant lines for RWA1 and RWA2. The study indicated that accessions in the National Small Grains Collection conferring RWA1 or RWA2 resistance comprise a diversified population which should support introgression efforts and provide genetic diversity for future breeding for RWA resistance.

• 出版日期2012-5