A new cellulose-producing bacterium, Rhodococcus sp MI 2: Screening and optimization of culture conditions

作者：Tanskul Som****^*; Amornthatree Korntip; Jaturonlak Nathakan

来源：Carbohydrate Polymers, 2013, 92(1): 421-428.

DOI：10.1016/j.carbpol.2012.09.017

• Bacterial cellulose
• Rhodococcus sp MI 2
• Manilkara zapota
• Static culture
• Agitated culture
• Stirred culture

A total of 59 bacterial strains were isolated from ripe fruits and vegetables and tested for their ability to produce cellulose. Only one identified as Rhodococcus sp. MI 2 based on its taxonomic characteristics and 165 rDNA sequence analysis. The glucose was the only product of digestion by cellulase confirmed by TLC and reversed phase HPLC. Rhodococcus sp. MI 2 produced significantly more cellulose with the SH medium containing glucose than with the coconut juice medium. Rhodococcus sp. MI 2 initially produced 3.91 +/- 0.091, 2.20 +/- 0.090 and 0.19 +/- 0.051 g/L/6 days cellulose under static, agitated and stirred conditions, respectively, whereas A. xylinum 998 produced 1.17 +/- 0.065, 1.34 +/- 0.115 and 0.12 +/- 0.046 g/L/6 days cellulose under the same conditions, respectively. The optimum culture conditions for cellulose production in SH medium were room temperature (25 degrees C), a 5% (v/v) inoculum, peptone 0.7%. yeast extract 0.9%, and sucrose 1.5%, at a pH of 3.5. The cellulose yield was increased by adding 0.5% CaCO3 to the SH medium with sucrose but added agar had no effect. The cellulose yield under optimum and static conditions was increased about twice, from 3.7 to 7.4 g/L/14 days.

• 出版日期2013-1-30