Design and Discovery of New Combinations of Mutant DNA Polymerases and Modified DNA Substrates

作者：Rosenblum Sydney L; Weiden Aurora G; Lewis Eliza L; Ogonowsky Alexie L; Chia Hannah E; Barrett Susanna E; Liu Mira D; Leconte Aaron M^*

来源：ChemBioChem, 2017, 18(8): 816-823.

DOI：10.1002/cbic.201600701

• DNA polymerases
• DNA
• modified DNA synthesis
• protein engineering

Chemical modifications can enhance the properties of DNA by imparting nuclease resistance and generating more-diverse physical structures. However, native DNA polymerases generally cannot synthesize significant lengths of DNA with modified nucleotide triphosphates. Previous efforts have identified a mutant of DNA polymeraseI from Thermus aquaticus DNA (SFM19) as capable of synthesizing a range of short, 2-modified DNAs; however, it is limited in the length of the products it can synthesize. Here, we rationally designed and characterized ten mutants of SFM19. From this, we identified enzymes with substantially improved activity for the synthesis of 2F-, 2OH-, 2OMe-, and 3OMe-modified DNA as well as for reverse transcription of 2OMe DNA. We also evaluated mutant DNA polymerases previously only tested for synthesis for 2OMe DNA and showed that they are capable of an expanded range of modified DNA synthesis. This work significantly expands the known combinations of modified DNA and Taq DNA polymerase mutants.

• 出版日期2017-4-18