Determination of free D-alanine in the human plasma by capillary electrophoresis with optical fiber light-emitting diode-induced fluorescence detection

作者：Zhao, SL^*; Wang, B; He, M; Bai, WL; Chen, L

来源：Analytica Chimica Acta, 2006, 569(1-2): 182-187.

DOI：10.1016/j.aca.2006.03.072

• capillary electrophoresis
• light-emitting diode
• D-alanine
• human plasma

A new analytical method for D-alanine (D-Ala) based on capillary electrophoretic separation and optical fiber light-emitting diode (LED)induced fluorescence detection has been developed. Fluorescein isothiocyanate (FITC) was used for precolumn derivatization of amino acids. The separation and determination of the derivatives was performed using a novel laboratory-built CE system with optical fiber LED-induced fluorescence detector. Optimal separation was performed at 15 kV using a background electrolyte solution consisted of 25 mmol L-1 sodium borate (pH 9.5), 18 mmol L-1 beta-CD and 15 mmol L-1 1 SDS. High sensitive detection was obtained by the optical fiber LED-induced fluorescence detection using a blue LED as the excitation source. The limits of detection (S/N= 3) for D-Ala was 8.0 x 10(-9) mol L-1. A calibration curve ranging from 2.0 x 10(-8) mol L-1 to 1.0 x 10(-6) mol L-1 was shown to be linear. Using this method, the levels Of D-Ala in human plasma from healthy donors were determined.

• 出版日期2006-5-31
• 单位广西师范大学