A Novel Magnetic Nanoparticle for Early Detection of Amyloid Plaques in Alzheimer's Disease

作者：Zeng, Jiaqi; Wu, Jiaqi; Li, Minghua; Wang, Peijun^*

来源：Archives of Medical Research, 2018, 49(4): 282-285.

DOI：10.1016/j.arcmed.2018.09.005

• Alzheimer's disease
• Amyloid plaques
• Pittsburgh compound B
• Magnetic nanoparticle
• In vitro binding

Early diagnosis of Alzheimer's disease (AD) remains challenging even with the assistance of imaging. Radiation exposure limits the application of positron emission tomography (PET) for amyloid imaging. Magnetic resonance imaging (MRI) offers superior spatial resolution without the disadvantage of radiation exposure. We developed Mn0.6Zn0.4Fe2O4 (MZF) modified by Pittsburgh compound B (PiB) to specifically bind to amyloid plaques. Coated with amphiphilic nanopolymer 6sPCL-b-P(MEO(2)MA-co-OEGMA), PiB-MZF was stable, biocompatible, and approximately 100 nm in size. The R(2 )relaxation rate of PiB-MZF was 169.93 mM(-1)S(-1) demonstrating excellent super-paramagnetism as a T(2 )negative contrast agent. PiB-MZF also showed no cytotoxicity in two cell lines. Immunohistochemistry indicated successful in vitro binding of PiB-MZF with A beta plaque on 6 months old AD mice brain sections. In conclusion, PiB-MZF nanoparticles are preliminarily proven to have the potential for early detection of amyloid plaques and diagnosis of AD.

• 出版日期2018-5
• 单位同济大学