Bone formation and degradation of a highly porous biphasic calcium phosphate ceramic in presence of BMP-7, VEGF and mesenchymal stem cells in an ectopic mouse model

作者：Roldan J C^*; Detsch R; Schaefer S; Chang E; Kelantan M; Waiss W; Reichert T E; Gurtner G C; Deisinger U

来源：Journal of Cranio-Maxillofacial Surgery, 2010, 38(6): 423-430.

DOI：10.1016/j.jcms.2010.01.003

• growth factors
• BMP-7
• VEGF
• mesenchymal stem cells
• calcium phosphate ceramic
• ectopic bone formation

Introduction: Angiogenesis and mesenchymal stem cells (MSCs) promote osteogenesis. The aim of the present study was to evaluate whether bone morphogenetic protein (BMP-7) promoted osteoinduction could be enhanced by combining it with vascular endothelial growth factor (VEGF) or MSCs in highly porous biphasic calcium phosphate (BCP) ceramics. Materials and methods: BCP ceramic blocks were implanted in an ectopic site in 24 mice (BMP-7 vs. BMP-7/MSCs and BMP-7 vs. Control; each group n = 8). Specimens were analysed 12 weeks after surgery by environmental scanning electron microscopy (ESEM) and Giemsa staining. Results: In all implanted scaffolds, newly formed bone was observed, even in the control site. No statistical differences in the amount of new bone were found in the presence of BMP-7 compared to BMP-7/VEGF (p = 1.0) or BMP-7/MSCs (p = 0.786). ESEM revealed a degradation of the scaffolds. A higher degradation was observed in areas where no bone-implant contact was present compared to areas where the ceramic was integrated in newly formed bone. Conclusions: Neither VEGF nor MSCs enhanced BMP-7 induced bone formation under the selected conditions. The present ceramic seed to be osteoinductive and degradable, making this material suitable for bone tissue engineering.

• 出版日期2010-9