Dual effect of benzyl alcohol on alpha-glucosidase activity: efficient substrate for high yield transglucosylation and non-competitive inhibitor of its hydrolytic activity

作者：Pavlovic Marija; Dimitrijevic Aleksandra^*; Bezbradica Dejan; Milosavic Nenad; Gavrovic Jankulovic Marija; Segan Dejan; Velickovic Dusan

来源：Carbohydrate Research, 2014, 387: 14-18.

DOI：10.1016/j.carres.2013.08.028

• Benzyl alcohol
• alpha-Glucosidase inhibition
• Transglucosylation
• Primary hydrolysis

Benzyl alcohol, a potent anesthetic and bacteriostatic, can be efficiently glucosylated by alpha-glucosidase from Saccharomyces cerevisiae to produce benzyl alcohol alpha-glucoside with a 75% yield. However, while studying the transglucosylation reaction conditions, it was found out that benzyl alcohol is a non-competitive inhibitor of alpha-glucosidase's hydrolytic activity (K-i = 18 mM, toward maltose). Due to its interesting ability to be glycosylated by the enzyme and to inhibit its hydrolytic activity, we proposed a plausible mechanism for the phenolic alpha-glucosydase inhibitor's binding, since the mechanism of inhibition has not yet been elucidated.

• 出版日期2014-3-31