A convenient 'click chemistry' approach to perylene diimide-oligonucleotide conjugates

作者：Ustinov Alexey V; Dubnyakova Veronika V; Korshun Vladimir A^*

来源：Tetrahedron, 2008, 64(7): 1467-1473.

DOI：10.1016/j.tet.2007.11.048

• modified oligonucleotides
• perylene diimide
• alkynes
• azides
• catalytic huisgen [3+2] cycloaddition

Terminal alkyne-containing phosphoramidite and solid support were prepared and used for the synthesis of 5'- and 3'-alkyne-modified oligonucleotides. The copper-catalyzed Huisgen [3+2] cycloaddition reaction in water-DMSO was used for the efficient preparation of perylene diimide-oligonucleotide conjugates suitable for constructing various types of PDI-modified DNA duplexes.

• 出版日期2008-2-11