A Common Clathrin-Mediated Machinery Co-ordinates Cell-Cell Adhesion and Bacterial Internalization

作者：Bonazzi Matteo^*; Kuehbacher Andreas; Toledo Arana Alejandro; Mallet Adeline; Vasudevan Lavanya; Pizarro Cerda Javier; Brodsky Frances M; Cossart Pascale

来源：Traffic, 2012, 13(12): 1653-1666.

DOI：10.1111/tra.12009

• E-cadherin
• adherens junctions
• actin
• internalin
• Listeria monocytogenes

Invasive bacterial pathogens often target cellular proteins involved in adhesion as a first event during infection. For example, Listeria monocytogenes uses the bacterial protein InIA to interact with E-cadherin, hijack the host adherens junction (AJ) machinery and invade non-phagocytic cells by a clathrin-dependent mechanism. Here, we investigate a potential role for clathrin in cell-cell adhesion. We observed that the initial steps of AJ formation trigger the phosphorylation of clathrin, and its transient localization at forming cell-cell contacts. Furthermore, we show that clathrin serves as a hub for the recruitment of proteins that are necessary for the actin rearrangements that accompany the maturation of AJs. Using an InIA/E-cadherin chimera, we show that adherent cells expressing the chimera form AJs with cells expressing E-cadherin. We demonstrate that non-adherent cells expressing the InIA chimera, as bacteria, can be internalized by E-cadherin-expressing adherent cells. Together these results reveal that a common clathrin-mediated machinery may regulate internalization and cell adhesion and that the relative mobility of one of the interacting partners plays an important role in the commitment to either one of these processes.

• 出版日期2012-12