APOBEC-1 and AID are nucleo-cytoplasmic trafficking proteins but APOBEC3G cannot traffic

作者：Bennett Ryan P; Diner Elie; Sowden Mark P; Lees Joshua A; Wedekind Joseph E; Smith Harold C^*

来源：Biochemical and Biophysical Research Communications, 2006, 350(1): 214-219.

DOI：10.1016/j.bbrc.2006.09.032

• APOBEC3G
• AID
• APOBEC-1
• trafficking
• NLS
• NES

Human APOBEC3G (hA3G) is a member of the APOBEC-1 related protein (ARP) family of cytidine deaminases. hA3G functions as a natural defense against endogenous retrotransposons and a multitude of retroviruses, most notably human immunodeficiency virus type I (HIV-1). Nothing is known about the cellular function of hA3G, however, upon HIV-1 infection hA3G functions as an antiviral factor by mutating viral single-stranded DNA during reverse transcription. Whereas homologous deaminases such as APOBEC-1 and AID act on RNA and DNA, respectively, in the cell nucleus, hA3G mutagenic activity appears to be restricted to the cytoplasm. We demonstrate that hA3G is not a nucleo-cytoplasmic shuttling protein like APOBEC-1 and AID, but is strongly retained in the cytoplasm through a mechanism that involves both the N and C-terminal regions of the protein.

• 出版日期2006-11-10