Mutational analysis and molecular modelling of an amino acid sequence motif conserved in antiporters but not symporters in a transporter superfamily

作者：Varela MF; Sansom CE; Griffith JK

来源：Molecular Membrane Biology, 1995, 12(4): 313-319.

DOI：10.3109/09687689509072433

• antiporter motif
• tetracycline resistance
• molecular modelling
• site-directed mutagenesis
• functional domains of antiport proteins

Elements of a 'G X(8) G X(3) G P X(2) G G' amino acid sequence motif were conserved in the fifth predicted membrane-spanning domains of 31 antiporters, but none of 27 symporters or uniporters that together comprise a 'superfamily' of structurally-related transport proteins. Molecular modelling and mechanics predicted that the GP dipeptide of this motif bends the antiporters' fifth transmembrane helices, and that the repeating pattern of glycine residues forms a pocket, devoid of side chains, on the surface of these helices. The glycine residue in the motif's GP dipeptide was conserved in 90% of these antiporters with alanine being the only observed substitution. Replacement of the glycine residue of the GP dipeptide with alanine and serine reduced the level of tetracycline resistance conferred by TetA(C), I a tetracycline/H+ antiporter, by 74 and 81%, respectively. All other substitutions totally abolished resistance to tetracycline. In cont

• 出版日期1995-12