Upregulation of Peroxisome Proliferator-Activated Receptor-γ and NADPH Oxidases Are Involved in Restenosis After Balloon Injury

作者：Qin, Hai-Dong; Huang, Dan; Weng, Xiao-Dan; Xu, Feng^*

来源：Journal of Cellular Physiology, 2009, 221(2): 387-393.

DOI：10.1002/jcp.21865

• SMOOTH-MUSCLE-CELLS
• ANGIOPLASTY-INDUCED SUPEROXIDE
• PPAR-GAMMA
• CORONARY-ARTERY
• NEOINTIMAL HYPERPLASIA
• ANGIOTENSIN-II
• INTIMAL HYPERPLASIA
• NAD(P)H OXIDASE
• CAROTID-ARTERY
• LIGAND

Restenosis is a major complication of percutaneous transluminal coronary angioplasty (PTCA) and is characterized by increased superoxide formation and accumulation of smooth muscle cells (SMCs). The mechanisms through which peroxisome proliferator-activated receptor-gamma (PPAR-gamma) modulates the pathological process are incompletely defined. In this study, balloon injury of porcine coronary arteries in vivo and cell scraping model in vitro were used to elucidate the pathway via this molecule. PPAR-gamma and NADPH oxidase expression significantly increased both in neointimal hyperplasia after balloon injury or in the cultured SMCs after scraping injury. In vitro, PPAR--y agonist I 5-deoxy-Delta(12,14)-prostagladlin J(2)(15d-PGJ2) decreased cell-scraping-induced superoxide generation through suppression of NADPH oxidase activity via down-regulation of p22(phox) and gp91(Phox). Furthermore, 15d-PGJ2 could suppress scraping-stimulated proliferation of SMCs. These data demonstrate that upregulation of PPAR-gamma and NADPH oxidases are involved in restenosis and activation of PPAR-gamma can inhibit the NADPH oxidase-dependent superoxide generation in SMCs after injury. These findings will provide a new potential drug target for restenosis after balloon injury. J. Cell. Physiol. 221: 387-393, 2009.

• 出版日期2009-11
• 单位复旦大学; 南京医科大学