Assessment of antibacterial properties and the active ingredient of plant extracts and its effect on the performance of crucian carp (Carassius auratus gibelio var. E'erqisi, Bloch)

作者：Lu Chunxia^*; Luo Xiaoling; Luo Ruifeng; Chen Xia; Xing Lijie; Tang Zonggui; Li Hongmin

来源：Journal of the Science of Food and Agriculture, 2013, 93(4): 902-909.

DOI：10.1002/jsfa.5823

• plant extracts
• UPLCMS
• MS
• Aeromona hydrophila
• antibacterial property
• crucian carp (Carassius auratus gibelio var
• E'erqisi
• Bloch)

BACKGROUND: In this study, the antibacterial properties and active ingredient of plant extracts and its effect on the performance of crucian carp (Carassius auratus gibelio var. E'erqisi, Bloch) were assessed. RESULTS: The transmission electron microscopy and flow cytometric analysis showed that the antibacterial activity of plant extracts is due to the disruption of the cell membrane and the leakage of cytoplasmic contents. The UPLCMS/MS analysis showed that the contents of gallic acid, ()epigallocatechin, (+)catechin, ()epigallocatechin gallate, ()epicatechin gallate, aloeemodin, rhein, emodin, chrysophanol, and physcion, were 5.27%, 3.30%, 1.08%, 19.32%, 5.46%, 0.23%, 0.56%, 1.28%, 0.75% and 0.39% in plant extracts, respectively. Results of feeding experiment showed that feeding crucian carp with 1.0% and 2.0% plant extracts significantly enhanced specific growth rate, serum total protein, lysozyme, catalase and superoxide dismutase activities, and decreased the feed conversion rate, malondialdehyde contents and the mortality rate (P < 0.05). CONCLUSION: It can be concluded that plant extracts added to fish feed can act as natural antimicrobial and immunostimulants to prevent pathogenic infection, enhance immune response, and promote growth of the fish.

• 出版日期2013-3-15
• 单位新疆农垦科学院