Double-Stranded RNA-Specific Templated Reaction with Triplex Forming PNA

作者：Kim Ki Tae; Chang Dalu; Winssinger Nicolas

来源：Helvetica Chimica Acta, 2018, 101(3): e1700295.

DOI：10.1002/hlca.201700295

<jats:p><jats:styled-content style="fixed-case">RNA</jats:styled-content>, originally perceived as a simple information transfer biopolymer, is emerging as an important regulator in cellular processes. A number of non‐coding <jats:styled-content style="fixed-case">RNA</jats:styled-content>s are double‐stranded and there is a need for technologies to reliably detect and image such <jats:styled-content style="fixed-case">RNA</jats:styled-content>s for biological and biomedical research. Herein we report double‐stranded <jats:styled-content style="fixed-case">RNA</jats:styled-content>‐specific templated reaction resulting from <jats:styled-content style="fixed-case">PNA</jats:styled-content>‐reagent conjugates that are brought within reactive distance through the formation of sequence‐specific triplexes onto double‐stranded <jats:styled-content style="fixed-case">RNA</jats:styled-content>. The reaction makes use of a ruthenium‐based photocatalyst that reduces a pyridinium‐based immolative linker, unmasking a profluorophore. The reaction was shown to proceed with signal amplification and to be selective for double‐stranded <jats:styled-content style="fixed-case">RNA</jats:styled-content> over <jats:styled-content style="fixed-case">DNA</jats:styled-content> as well as single‐stranded <jats:styled-content style="fixed-case">RNA</jats:styled-content>. The generality of the triplex formation was enabled by non‐canonical nucleobases that extend the <jats:italic>Hoogsteen</jats:italic> base‐pairing repertoire. The technology was applied to a templated reaction using pre‐micro<jats:styled-content style="fixed-case">RNA</jats:styled-content> 31.</jats:p>

• 出版日期2018-3