Assembly of a protein "brush" by end-grafting titin fragments to liposomes

作者：Chami Mohamed; Franke Barbara; Mayans Olga; Kreplak Laurent^*

来源：Journal of Bioscience and Bioengineering, 2011, 112(2): 178-179.

DOI：10.1016/j.jbiosc.2011.04.014

• Decorated liposomes
• Titin
• Cryo-electron microscopy
• His-Tag
• Nickel chelating lipid

Titin is a giant filamentous protein of striated muscle composed of >300 immunoglobulin-like domains linked in tandem. Here, we demonstrate that a six-immunoglobulin fragment of titin carrying a poly-histidine tag forms a protein "brush" on liposomes containing metallochelating lipids. These specimens might allow the direct visualization by cryo-EM of frozen hydrated and unstained titin chains with preserved architectural features.

• 出版日期2011-8