A Switch in Hepatic Cortisol Metabolism across the Spectrum of Non Alcoholic Fatty Liver Disease

作者：Ahmed Adeeba^*; Rabbitt Elizabeth; Brady Theresa; Brown Claire; Guest Peter; Bujalska Iwona J; Doig Craig; Newsome Philip N; Hubscher Stefan; Elias Elwyn; Adams David H; Tomlinson Jeremy W; Stewart Paul M

来源：PLos One, 2012, 7(2): e29531.

DOI：10.1371/journal.pone.0029531

Context: Non alcoholic fatty liver disease (NAFLD) is the hepatic manifestation of the metabolic syndrome. NAFLD represents a spectrum of liver disease ranging from reversible hepatic steatosis, to non alcoholic steato-hepatitis (NASH) and cirrhosis. The potential role of glucocorticoids (GC) in the pathogenesis of NAFLD is highlighted in patients with GC excess, Cushing%26apos;s syndrome, who develop central adiposity, insulin resistance and in 20% of cases, NAFLD. Although in most cases of NAFLD, circulating cortisol levels are normal, hepatic cortisol availability is controlled by enzymes that regenerate cortisol (F) from inactive cortisone (E) (11 beta-hydroxysteroid dehydrogenase type 1, 11 beta-HSD1), or inactivate cortisol through A-ring metabolism (5 alpha- and 5 beta-reductase, 5 alpha R and 5 beta R). %26lt;br%26gt;Objective and Methods: In vitro studies defined 11 beta-HSD1 expression in normal and NASH liver samples. We then characterised hepatic cortisol metabolism in 16 patients with histologically proven NAFLD compared to 32 obese controls using gas chromatographic analysis of 24 hour urine collection and plasma cortisol generation profile following oral cortisone. %26lt;br%26gt;Results: In patients with steatosis 5 alpha R activity was increased, with a decrease in hepatic 11 beta-HSD1 activity. Total cortisol metabolites were increased in this group consistent with increased GC production rate. In contrast, in patients with NASH, 11 beta-HSD1 activity was increased both in comparison to patients with steatosis, and controls. Endorsing these findings, 11 beta-HSD1 mRNA and immunostaining was markedly increased in NASH patients in peri septal hepatocytes and within CD68 positive macrophages within inflamed cirrhotic septa. %26lt;br%26gt;Conclusion: Patients with hepatic steatosis have increased clearance and decreased hepatic regeneration of cortisol and we propose that this may represent a protective mechanism to decrease local GC availability to preserve hepatic metabolic phenotype. With progression to NASH, increased 11 beta-HSD1 activity and consequent cortisol regeneration may serve to limit hepatic inflammation.

• 出版日期2012-2-20