Binding of a monoclonal antibody positively correlates with bioactivity of the F4 fimbrial adhesin FaeG associated with post-weaning diarrhoea in piglets

作者：Verdonck F^*; Snoeck V; Goddeeris BM; Cox E

来源：Journal of Immunological Methods, 2004, 294(1-2): 81-88.

DOI：10.1016/j.jim.2004.08.007

• Escherichia coli
• F4 (K88) fimbriae
• bioactivity
• receptor
• adhesin

Piglets are susceptible to F4 (K88)(+) enterotoxigenic Escherichia coli (ETEC)-induced neonatal and post-weaning diarrhoea. The F4 fimbriae are composed of some minor subunits and the major subunit FaeG that also constitutes the adhesin. Parenteral vaccination of sows with an F4-containing vaccine protects the suckling piglets against neonatal F4(+) ETEC-induced diarrhoea, but no commercial (mucosal) vaccine exists against F4(+) ETEC-induced weaning diarrhoea. To develop a vaccine, a bioactive F4-receptor (F4R) binding FaeG molecule is required that binds to the F4R following oral immunization and induces a FaeG-specific immune response. The present study reports the altered binding of the FaeG-specific monoclonal antibody IMM01 with bioactive versus non-bioactive F4 fimbrial adhesin FaeG. The correlation of altered IMM01 binding with altered FaeG bioactivity permits the use of an IMM01-based ELISA as a fast, specific and sensitive in vitro selection for potent F4 or (recombinant) FaeG antigen formulations, useful in an F4(+) ETEC vaccine.

• 出版日期2004-11