Detection of unusual rotavirus genotypes G8P[8] and G12P[6] in South Korea

作者：Le Van Phan; Kim Ji Yeon; Cho Sung Lim; Nam Sun Woo; Lim Inseok; Lee Hee Joong; Kim Kijeong; Chung Sanf In; Song Wonkeun; Lee Kyu Man; Rhee Moon Soo; Lee Jung Sook; Kim Wonyong^*

来源：Journal of Medical Virology, 2008, 80(1): 175-182.

DOI：10.1002/jmv.21044

• diarrhea
• ELISA
• RT-PCR
• G type
• P type

Five hundred four fecal specimens, collected between 2004 and 2006 from young children with acute diarrhea, were screened for rotavirus by ELISA with VP6-specific antibody. Of these samples, 394 (78.2%) were confirmed as group A rotavirus and they underwent G- and P typing using a combination of ELISA, RT-PCR, and sequence analysis methods. The dominant circulating G serotype was G1 (35.6%) followed by G3 (26.4%), G4 (14.7%), and G2 (11.9%). There was a low prevalence of G9 (1.0%) and of unusual G type rotavirus, in particular, G12 (0.5%) and G8 (0.3%). Of the P genotype rota virus in circulation, P[8](53.0%) was most common followed by P[6] (15.5%), P[4] (15.2%), and P[91 (2.3%). Determination of G- and P type combinations revealed that G1P[8] strains were most prevalent (25.4%), amid G3P[8] (16.8%), G2P[4] (6.3%), and G4P[6] (6.1%) strains. Unusual or rare combinations such as G2P[6], G2P[8], G3P[4], G2P[9], G1P[9], G3P[9], G12P[6], G1P[4], G3P[6], and G8P[8] were also found. Owing to the recent emergence of G8 and G12 rotavirus, the findings from this study are important since they provide new information concerning the local and global spread of rotavirus genotypes.

• 出版日期2008-1