Monitoring therapeutic effects in experimental stroke by serial USPIO-enhanced MRI

作者：Marinescu Marilena; Chauveau Fabien; Durand Anne; Riou Adrien; Cho Tae Hee; Dencausse Anne; Ballet Sebastien; Nighoghossian Norbert; Berthezene Yves; Wiart Marlene^*

来源：European Radiology, 2013, 23(1): 37-47.

DOI：10.1007/s00330-012-2567-2

• MRI
• USPIO
• Inflammation
• Cerebral ischaemia
• Treatment monitoring

This study sought to evaluate whether the therapeutic effects of an anti-inflammatory drug such as minocycline could be monitored by serial ultrasmall superparamagnetic particles of iron oxide (USPIO)-enhanced MRI in experimental stroke. %26lt;br%26gt;Mice received a three-dose minocycline treatment (n = 12) or vehicle (n = 12) after permanent middle cerebral artery occlusion. USPIOs were administered 5 h post-surgery. MRI was performed before, 24 h and 48 h post-USPIO administration. MRI endpoints were the extent of signal abnormalities on R2 maps (=1/T2) and quantitative R2 changes over time (a dagger R2). Post-mortem brains were prepared either for immunohistology (n = 16) or for iron dosage (n = 8). %26lt;br%26gt;As expected, treatment with minocycline significantly reduced infarct size, blood-brain barrier permeability and F4/80 immunostaining for microglia/macrophages. Areas of R2 maps %26gt; 35 ms(-1) also appeared significantly decreased in minocycline-treated mice (ANOVA for repeated measures, P = 0.017). There was a fair correlation between these areas and the amount of iron in the brain (R-2 = 0.69, P = 0.010), but no significant difference in a dagger R2 was found between the two groups. %26lt;br%26gt;This study showed that the extent of signal abnormalities on R2 maps can be used as a surrogate marker to detect minocycline effects in a murine experimental model of stroke. %26lt;br%26gt;aEuro cent Ultrasmall superparamagnetic particles of iron oxide offer new avenues for MRI research %26lt;br%26gt;aEuro cent Treatment of the inflammatory response following ischaemic stroke is currently undergoing evaluation. %26lt;br%26gt;aEuro cent Minocycline treatment significantly reduced areas of signal abnormalities on USPIO-enhanced MRI. %26lt;br%26gt;aEuro cent These areas correlated with the amount of iron in the brain. %26lt;br%26gt;aEuro cent Thus USPIO-enhanced MRI might provide a surrogate marker to monitor treatment.

• 出版日期2013-1