科研之友
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摘要
The selective detection of Cu2+ and S2- in pure water systems is very challenging. To address this problem, we here present a novel fluorescent probe CF536 for the reversible detection of Cu2+ and S2- in a 100% aqueous solution via a displacement approach based on fluorescence quenching and the ESIPT mechanism, and characterized by IR, NMR, HRMS and X-ray analysis. The introduction of Cu2+ (1.0 equiv.) to the aqueous solution of CF536 led to quenching of the intense green fluorescence within 2 min. On the addition of S2- (3.0 equiv.), the emission of the ESIPT probe soon recovered from the CF536-Cu2+ system due to the abolishment of paramagnetic Cu2+. The fluorescence "ON-OFF-ON" cycle can be repeated a minimum of 5 times by the alternate addition of Cu2+ and S2-, implying that CF536 is a renewable dual-functional probe. The probe displayed high selectivity, a rapid response time, excellent water solubility due to introduction of the extremely hydrophilic trihydroxyl group and outstanding anti-interference ability for its large Stokes shift of 101 nm (lambda(abs) = 435 nm, lambda(em) = 536 nm), thus making the method highly suitable for bioimaging. The probe CF536 was successfully used for fluorescent imaging of copper and hydrogen sulfide in living cells.
