Anti-hypertensive azelnidipine preserves insulin signaling and glucose uptake against oxidative stress in 3T3-L1 adipocytes

作者：Tatsumi Fuminori^*; Kaneto Hideaki; Hashiramoto Mitsuru; Tawaramoto Kazuhito; Obata Atsushi; Kimura Tomohiko; Shimoda Masashi; Hamamoto Sumiko; Kanda Kimura Yukiko; Kamei Shinji; Mune Tomoatsu; Matsuda Masafumi; Kaku Kohei

来源：Endocrine Journal, 2015, 62(8): 741-747.

DOI：10.1507/endocrj.ej15-0273

• Azelnidipine
• Insulin signaling
• Glucose uptake
• Oxidative stress
• Inflammatory cytokine

It is known that reactive oxygen species (ROS) are involved in the development of insulin resistance as well as pancreatic beta-cell dysfunction both of which are often observed in type 2 diabetes. In this study, we evaluated the effects of azelnidipine, a calcium channel blocker, on ROS-mediated insulin resistance in adipocytes. When 3T3-L1 adipocytes were exposed to ROS, insulin-mediated glucose uptake was suppressed, but such phenomena were not observed in the presence of azelnidipine. Phosphorylation of insulin receptor and phosphorylation of Akt were suppressed by ROS, which was mitigated by azelnidipine treatment. Activation of the INK pathway induced by ROS was also reduced by azelnidipine. Various inflammatory cytokine levels were increased by ROS, which was also suppressed by azelnidipine treatment. In contrast, adiponectin mRNA and secreted adiponectin levels were reduced by ROS, which was refilled by azelnidipine treatment. In conclusion, azelnidipine preserves insulin signaling and glucose uptake against oxidative stress in 3T3-L1 adipocytes.

• 出版日期2015-8-20