A Dual Radiologic Contrast Agent Protocol for F-18-FDG and F-18-FLT PET/CT Imaging of Mice Bearing Abdominal Tumors

作者：Aide Nicolas^*; Kinross Kathryn; Beauregard Jean Mathieu; Neels Oliver; Potdevin Titaina; Roselt Peter; Dorow Donna; Cullinane Carleen; Hicks Rodney J

来源：Molecular Imaging and Biology, 2011, 13(3): 518-525.

DOI：10.1007/s11307-010-0378-x

• PET/CT
• Preclinical studies
• Abdominal tumors
• Contrast media

The aim of the study was to improve abdominal tumor detection by use of a dual radiologic contrast protocol.
eXia160A (R) (Benitio international) was mixed with 2-deoxy-2-[F-18]fluoro-d-glucose or 3'-[F-18]fluoro-3'-deoxythymidine for intravenous (IV) injections. OmnipaqueA (R) 300 (GE healthcare) was used for intraperitoneal (IP) injections. Positron emission tomography/computed tomography (PET/CT) scans were acquired on a Siemens BiographA (R) equipped with point spread function reconstruction. The optimal concentration and injection schedule of IP contrast agent was studied in 12 mice. The impact of IP contrast media on PET quantitative accuracy was investigated by phantom studies and by imaging six mice before and after IP injection of OmnipaqueA (R). The impact of a dual contrast media protocol on tumor delineation and quantitation was evaluated in 15 tumor-bearing mice using ex vivo counting as the reference.
The optimal sequence was a mixture of tracer plus IV contrast agent followed by 1 mL of IP contrast agent (20 mg iodine/mL) administered 10 min before PET/CT acquisition. Phantom studies showed that the use of a 20-mg iodine/mL concentration of OmnipaqueA (R) led to a 4.8% overestimation of radioactivity concentration, as compared to saline. This was confirmed by animal studies that demonstrated a 4.3% overestimation. Tumor detection was excellent and correlation between PET/CT quantitative data and ex vivo counting was good (r (2) = 0.91, slope = 0.7).
A dual radiologic contrast protocol is useful in PET/CT scanning of mice bearing abdominal tumors. Contrast agents used in this manner lead to a small but acceptable overestimation of quantitative PET data.

• 出版日期2011-6