Direct detection of aptamer-thrombin binding via surface-enhanced Raman spectroscopy

作者：Pagba Cynthia V^*; Lane Stephen M; Cho Hansang; Wachsmann Hogiu Sebastian

来源：Journal of Biomedical Optics, 2010, 15(4): 047006.

DOI：10.1117/1.3465594

• aptamer
• quadruplex
• label-free assay
• direct detection
• thrombin
• surface-enhanced Raman Scattering

In this study, we exploit the sensitivity offered by surface-enhanced Raman scattering (SERS) for the direct detection of thrombin using the thrombin-binding aptamer (TBA) as molecular receptor. The technique utilizes immobilized silver nanoparticles that are functionalized with thiolated thrombin-specific binding aptamer, a 15-mer (5'-GGTTGGTGTGGTTGG-3') quadruplex forming oligonucleotide. In addition to the Raman vibrational bands corresponding to the aptamer and blocking agent, new peaks (mainly at 1140, 1540, and 1635 cm(-1)) that are characteristic of the protein are observed upon binding of thrombin. These spectral changes are not observed when the aptamer-nanoparticle assembly is exposed to a nonbinding protein such as bovine serum albumin (BSA). This methodology could be further used for the development of label-free biosensors for direct detection of proteins and other molecules of interest for which aptamers are available.

• 出版日期2010-8
• 单位国家自然科学基金委员会