科研之友
微信
新浪微博
Facebook
分享链接
摘要
A method involving LC coupled with MS/MS (LC/MS/MS) was designed for simultaneous quantification of 10 antifungal drugs (voriconazole, griseofulvin, clotrimazole, bifonazole, econazole, ketoconazole, itraconazole, miconazole, terconazole, and fluconazole) in the liver and muscles of chickens. Homogenized tissue samples were extracted with acetonitrile and subsequently underwent freezing-delipidation. A Waters Acquity Ultra Performance LC BEH C18 column was used to separate the analytes, coupled with MS/MS using an electrospray ionization source. The accuracy of the method was confirmed with a mean recovery of 71-121%, and acceptable coefficients of variation (4-23%, n = 6). The detection capability of these compounds in two different matrixes was 0.50-2.82 mu g/kg. This method can be applied for the screening and confirmation of target antifungal drugs in chicken tissues.
- 出版日期2011-9
- 单位中国疾病预防控制中心; 中国科学院长春应用化学研究所; 南昌大学; 北京化工大学
