Metal-Polymer Hybrid Nanoparticles for Correlative High-Resolution Light and Electron Microscopy

作者：Reifarth Martin; Preussger Elisabeth; Schubert Ulrich S^*; Heintzmann Rainer^*; Hoeppener Stephanie^*

来源：Particle & Particle Systems Characterization, 2017, 34(10): 1700180.

DOI：10.1002/ppsc.201700180

• correlative light and electron microscopy
• gold nanoparticles
• poly(ethylene imine)
• scanning transmission electron microscopy
• structured illumination microscopy
• uptake studies

The combination of fluorescence microscopy and electron microscopy promises a deeper insight into the ultrastructural features of cell organelles, e.g., after drug administration. Both methods complement each other and provide, as a correlative approach, a keen insight into the fate of nanoparticles within the cell. Moreover, it represents a promising tool to determine alterations of the cellular environment as a response to particle uptake. However, the availability of suitable correlative markers is mandatory for such correlative approaches. In this contribution, the utilization of poly(ethylene imine) based metal-polymer hybrid particles labeled with small gold nanoparticles and Rhodamine B facilitating the observation of the particles by means of fluorescence as well as by transmission electron microscopy is suggested. Correlative light and electron microscopy is used to study uptake and intracellular fusion processes of endosomal/lysosomal structures.

• 出版日期2017-10