A novel three primers PCR (TP-PCR) method to obtain recombinant DNA molecule independent of restriction enzyme

作者：Deng CY; Song GS; Xu JW; Zhu Z^*

来源：Chinese Science Bulletin, 2002, 47(24): 2067-2070.

DOI：10.1360/02tb9448

• DNA recombination
• TP-PCR
• cowpea trypsin inhibitor

In this note, we report a novel and efficient three primers PCR (TP-PCR) method to rapidly generate recombinant DNA molecule at precise junction between two arbitrary DNA fragments. TP-PCR method is characterized by its reaction system with two templates and three primers, which can produce a recombinant DNA molecule in one PCR reaction. The main advantages of this method are the independence of sequences at the recombination site, the rapidness, and the easy establishment of adequate conditions. This method has been successfully applied to constructing a fusion protein gene, sck gene.

• 出版日期2002-12
• 单位中国科学院遗传与发育生物学研究所