Adsorption behaviors of avian immunoglobulins and purification of immunoglobulin Y from chicken serum with mixed-mode resins

作者：Luo, Yingdi; Zhang, Qilei; Yao, Shanjing; Lin, Dongqiang^*

来源：Chinese Journal of Chemical Engineering, 2019, 27(3): 514-518.

DOI：10.1016/j.cjche.2018.05.011

• Mixed-mode chromatography
• Immunoglobulin Y
• Adsorption selectivity
• Separation

The importance of immunoglobulin Y (IgY) as a specific antibody equivalent to mammalian immunoglobulin G (IgG) is well recognized. However, production of highly purified IgY is still difficult due to the lack of specific purification methods. In this study, adsorption behaviors of IgY on four mixed-mode resins with functional ligands of 4-mercatoethyl-pyridine (MEP), 2-mercapto-1-methyli-midazole (MMI), 5-aminobenzi-midazole (ABI) and tryptophan-5-aminobenzi-midazole (W-ABI) were evaluated. The results showed that high adsorption ratio were found at pH 6.0-7.0 with little adsorption under acidic conditions. The resin with ABI ligand was then used to separate IgY from immunized chicken serum. An efficient process with IgY purity of 95% and recovery of 90% was developed after optimization of loading and elution pH and injection volume. The biological activity of the purified IgY was fully maintained. These results indicated that mixed-mode chromatography with specially-designed ligands has great potential for the separation of IgY from crude feedstock.

• 单位
  浙江大学