A simple and rapid method for visualization of male meiotic chromosomes in Arabidopsis thaliana

作者:Li, Junhua*; Yuan, Jinhong; Zhao, Xiting; Zhang, Xiaoli; Li, Mingjun
来源:Bioscience Biotechnology and Biochemistry, 2014, 78(6): 1026-1028.
DOI:10.1080/09168451.2014.905191

摘要

Meiotic chromosomes are of basic interest to the geneticist and cell biologist who study their behavior. A rapid and highly repeatable method for visualization of meiotic chromosomes is useful. Here we describe a fast staining protocol for Arabidopsis male meiotic chromosomes. Meiocytes were squashed into a labeling buffer, the chromosome morphology could be analyzed using fluorescence without any additional treatment.