Uruburuella testudinis sp nov., isolated from tortoise (Testudo)

作者：Kuhnert Peter^*; Thomann Andreas; Brodard Isabelle; Haefeli Willi; Korczak Bozena M

来源：International Journal of Systematic and Evolutionary Microbiology, 2015, 65(4): 1251-1255.

DOI：10.1099/ijs.0.000089

A polyphasic taxonomic analysis was carried out on 11 uncommon Gram-stain-negative, non-motile, catalase- and oxidase-positive, but indole-negative, bacterial strains isolated from tortoises. Phenotypically and genetically they represented a homogeneous group of organisms most closely related to, but distinct from, Uruburuella suis. In a reconstructed 16S rRNA gene tree they clustered on a monophyletic branch next to U. suis with gene:similarities between strains of 99.5-100%, and of up to 98.2% with U. suis. DNA-DNA hybridization indicated the organisms represented a novel species with only 40% DNA-DNA similarity with U. suis. Partial sequencing of rpoB resulted in two subclusters confirming the 16S rRNA gene phylogeny; both genes allowed clear separation and identification of the novel species. Furthermore, they could be unambiguously identified by matrix-assisted laser desorption ionization time-of-flight MS, where, again, they formed a highly homogeneous cluster separate from U. suis and other members of the family Neisseriaceae. The major fatty acids were C-16:0 and summed feature C-16:1 omega 7C/isO-C-15:0 2-OH. The DNA G+C content was 54.4 mol%. Based on phenotypic and genetic data we propose classifying these organisms as representatives of a novel species named Uruburuella testudinis sp. nov. The type strain is 07_0D624(T) (=DSM 26510(T)=CCUG 63373(T)).

• 出版日期2015-4