NF-κB inhibition rescues Toll-like receptor 9 expression in human papillomavirus type 11 infected HaCaT cells

作者：Ying, Zuolin^*; Wu, Zhouwei; Li, Xiaojie; Dang, Hong; Yin, Na; Gao, Chuang

来源：International Journal of Clinical and Experimental Pathology, 2017, 10(9): 9460-9467.

• Condyloma acuminatum
• HPV11
• NF-kappa B
• HaCaT

Objectives: Toll-like receptors (TLRs) are related to human papillomavirus (HPV) infections including condyloma acuminatum (CA). This study was designed to investigate the mechanism of TLR9 and nuclear factor kappa B (NF-kappa B) in CA. Methods: Expression of TLR9 protein in CA patients was detected and compared with those in CA relapse-free (CaRF) patients and normal control. HaCaT cells were transfected with HPV11 genome and NF-kappa B p65 siRNA or I kappa B kinase inhibitor BMS345541. Expression of NF-kappa B and TLR9 were detected using both PCR and Western blot methods. Results: TLR9 was downregulated in CA specimens as compared to CaRF and normal controls. HPV11 transfection into HaCaT (HPV11. HaCaT) cells reduced TLR9 expression and activated NF-kappa B p65 expression. However, administration of NF-kappa B p65 siRNA or I.B kinase inhibitor BMS345541 significantly inhibited NF-kappa B p65 expression and rescued the expression of TLR9. Conclusion: Inhibition of NF-kappa B activation could rescue TLR9 expression in HPV11. HaCaT cells. TLR9/NF-kappa B mechanism may provide new target for clinical treatment of CA.

• 出版日期2017
• 单位上海交通大学