Aggregation-induced conformational transitions in bovine beta-lactoglobulin adsorbed onto open chitosan structures

作者：Borges Joao; Campina Jose M^*; Souza Hileia K S; Goncalves Maria P; Fernando Silva A

来源：Soft Matter, 2012, 8(4): 1190-1201.

DOI：10.1039/c1sm06428b

Chitosan is a natural polysaccharide which strongly interacts with whey proteins in solution. Certain protein-polyelectrolyte complexes and electrostatically assembled thin films have been shown to be good platforms for the preservation of globular and small proteins in their native state mainly due to the hydrophilic nature of these polyelectrolytes and to the lack of physical space for embedded proteins to relax. As a consequence, the use of natural polyelectrolytes in new nanocomposite materials for medicine, chemical analysis, catalysis, etc. has exploded in the last few years. Nevertheless, in many of these applications proteins are immobilized in more open structures without physical restrictions to undergo conformational changes. In this work, we investigate the nature of these transitions for a model whey protein, beta-LG, on a chitosan-decorated Au surface in a scenario for which protein-surface interactions compete with boosted protein-protein non-coulombic forces. The adsorption kinetics, protein mass uptake (plus associated water), and flexibility of the adsorbed layers have been followed in situ by the quartz crystal microbalance with dissipation monitoring (QCM-D). Further ex situ characterization has been performed by atomic force microscopy (AFM) and non-invasive scanning electron microscopy (SEM). The balance between both types of interactions yielded surfaces heavily loaded with protein and water in which orientational transitions seemed restricted. The kinetics of the process was registered in a wide range of concentrations and successfully fitted to a double exponential equation derived from the RSA theory accounting for the establishment of slow post-adsorption conformational transitions.

• 出版日期2012