Dunaliella sp a Wild Algal Strain Isolated from the Sfax-Tunisia Solar Evaporating Salt-Ponds, a High Potential for Biofuel Production Purposes

作者：Chtourou Haifa; Dahmen Ines; Hassairi Ilem; Abdelkafi Slim; Sayadi Sami; Dhouib Abdelhafidh^*

来源：Journal of Biobased Materials and Bioenergy, 2014, 8(1): 27-34.

DOI：10.1166/jbmb.2014.1403

• Microalgae
• Dunaliella sp.
• Lipids
• Biodiesel

Countries lacking fresh water sources such as Tunisia, must use the sea water and the non-arable lands for the culture of microalgae to produce biofuel and overcome the national energy demand. In this framework, a green microalgae CTM 20032, was isolated from the coastal solar saltern at Sfax, Tunisia and was tested for its ability to accumulate lipid under autotrophic growth condition. The isolate was identified as Dunaliella sp. on the basis of 18S rDNA analysis. The dry weight of Dunaliella sp. was composed of lipids 100 mg g(-1), proteins 435 mg g(-1), carbohydrates 300 mg g(-1), chlorophylls 38.8 mg g(-1), carotenes 18 mg g(-1) and polyphenols 83 mg g(-1). The lipid GC-FID analysis showed that main fatty acids are Octadecanoic acid C18:0 48%, Hexadecatrienoic acid 16:3 40% and Octadecatrienoic acid 18:3 7.5%. Nitrogen and phosphorus depletions during 6 culture days resulted in Dunaliella sp. lipid content increase up to 54 and 45% (dw) respectively. Under nitrogen starvation, Dunaliella sp. fatty acid pattern is typical of the Dunaliella genus and mainly composed of 58% of C16:0, 20.5% of C18:0 and 15% of C18:1. Whereas under phosphorus starvation, its profile of fatty acids was mainly represented by 80% of C16:0, 8.5% of C18:0 and 12.5% of C18:1. Stressful conditions drive Dunaliella sp. to accumulate up to 95% of its lipids under form of saturated and monounsaturated triglycerides form and thereby being suitable for biodiesel production.

• 出版日期2014-2