X-ray diffraction from intact tau aggregates in human brain tissue

作者：Landahl, Eric C; Antipova, Olga; Bongaarts, Angela; Barrea, Raul; Berry, Robert; Binder, Lester I; Irving, Thomas; Orgel, Joseph; Vana, Laurel; Rice, Sarah E^*

来源：Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment , 2011, 649(1): 184-187.

DOI：10.1016/j.nima.2011.01.059

• X-ray diffraction
• Tau
• Alzheimer's disease
• Pick's disease

We describe an instrument to record X-ray diffraction patterns from diseased regions of human brain tissue by combining an in-line visible light fluorescence microscope with an X-ray diffraction microprobe. We use thiazine red fluorescence to specifically label and detect the filamentous tau protein pathology associated with Pick's disease, as several laboratories have done previously. We demonstrate that thiazine red-enhanced regions within the tissue show periodic structure in X-ray diffraction, which is not observed in healthy tissue. One observed periodicity (4.2 angstrom) is characteristic of cross-beta sheet structure, consistent with previous results from powder diffraction studies performed on purified, dried tau protein.

• 出版日期2011-9-1
• 单位西北大学