Dual Enzyme-Triggered In Situ Crosslinkable Gelatin Hydrogels for Artificial Cellular Microenvironments

作者：Kim Bae Young; Lee Yunki; Son Joo Young; Park Kyung Min; Park Ki Dong^*

来源：Macromolecular Bioscience, 2016, 16(11): 1570-1576.

DOI：10.1002/mabi.201600312

• artificial extracellular matrices
• enzyme-mediated crosslinking reaction
• glucose oxidase
• horseradish peroxidase
• in situ crosslinkable hydrogels

Horseradish peroxidase (HRP) and hydrogen peroxide (H2O2)-mediated crosslinking reaction has become an attractive method to create in situ forming hydrogels. While the crosslinking system has been widely utilized, there are certain issues require improvement to extend their biomedical applications, including creation of stiff hydrogels without compromising cytocompatibility due to initially high concentrations of H2O2. A gelatin-based hydrogels formed through a dual enzyme-mediated crosslinking reaction using HRP and glucose oxidase (GOx) as an H2O2-generating enzyme to gradually supply a radical source in HRP-mediated crosslinking reaction is reported. The physicochemical properties can be controlled by varying enzyme concentrations. Furthermore the hydrogel matrices provide 3D microenvironments for supporting the growth and spreading of human dermal fibroblasts with minimized cytotoxicity, despite the cells being encapsulated within stiff hydrogels. These hydrogels formed with HRP/GOx have great potential as artificial microenvironments for a wide range of biomedical applications.

• 出版日期2016-11