科研之友
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摘要
Power-time curves of growing recombinant Escherichia coli B1 cell suspensions, treated with different concentrations of Mg2+, were recorded by microcalorimeter. The extent of the stimulatory effect of Mg2+ on the growth of recombinant E. coli B1 was compared by reference to the changes in the values of the growth rate coefficient of bacteria (k), the time of reaching the maximum effect in the log phase (t(D)), the time of maintaining the maximum effect in the stationary period (t(S)), and the maximum thermal power during the entire bacterial growth (P-m) at different Mg2+ doses and the optimal Mg2+ dose was calculated. The experimental results revealed that when Mg2+ concentration reached gamma = 2.2 mg mL(-1), the stimulatory effect is the greatest. With more Mg2+ (gamma > 2.2 mg mL(-1)) added, the promotive effect would decrease drastically.
- 出版日期2008-9
- 单位浙江大学宁波理工学院; 浙江大学; 西北工业大学; 上海市计划生育科学研究所; 西北大学
