Development and characterization of microsatellite markers in Schizopyge niger (Family: Cyprinidae, Pisces) from Kashmir Valley

作者：Barat Ashoktaru; Matura Rakesh; Sati Jyoti; Kumar Rohit; Bhat Farroz A; Goel Chirag; Sahoo Prabhati Kumari

来源：Conservation Genetics Resources, 2013, 5(3): 831-833.

DOI：10.1007/s12686-013-9920-x

• Schizopyge niger
• Microsatellite marker
• Partial genomic library
• Conservation genetics

A set of 8 microsatellites was developed for a freshwater fish chush snowtrout, Schizopyge niger. Chush snowtrout is an endemic fish to Kashmir valley, India. Constructing a partial genomic library, we obtained 36 clones containing putative microsatellite motifs and 12 microsatellite with enough flanking sequence for primer designing. The number of alleles per locus ranged from 3 to 7 (mean 4.75), with a mean expected and observed heterozygosity of 0.71 and 0.68, respectively. All the loci were in Hardy-Weinberg equilibrium. There was no significant evidence for linkage disequilibrium between any pair of loci. These microsatellites were highly polymorphic (mean PIC 0.63) and will be useful for genetic variability and conservation genetics studies.

• 出版日期2013-9