Alcohol dehydrogenase gene ADH3 activates glucose alcoholic fermentation in genetically engineered Dekkera bruxellensis yeast

作者：Schifferdecker Anna Judith^*; Siurkus Juozas; Andersen Mikael Rordam; Joerck Ramberg Dorte; Ling Zhihao; Zhou Nerve; Blevins James E; Sibirny Andriy A; Piskur Jure; Ishchuk Olena P^*

来源：Applied Microbiology and Biotechnology, 2016, 100(7): 3219-3231.

DOI：10.1007/s00253-015-7266-x

• Dekkera bruxellensis
• Auxotrophic mutants
• Promoters
• TEF1
• Gene expression
• ADH3
• Glucose fermentation

Dekkera bruxellensis is a non-conventional Crabtree-positive yeast with a good ethanol production capability. Compared to Saccharomyces cerevisiae, its tolerance to acidic pH and its utilization of alternative carbon sources make it a promising organism for producing biofuel. In this study, we developed an auxotrophic transformation system and an expression vector, which enabled the manipulation of D. bruxellensis, thereby improving its fermentative performance. Its gene ADH3, coding for alcohol dehydrogenase, was cloned and overexpressed under the control of the strong and constitutive promoter TEF1. Our recombinant D. bruxellensis strain displayed 1.4 and 1.7 times faster specific glucose consumption rate during aerobic and anaerobic glucose fermentations, respectively; it yielded 1.2 times and 1.5 times more ethanol than did the parental strain under aerobic and anaerobic conditions, respectively. The overexpression of ADH3 in D. bruxellensis also reduced the inhibition of fermentation by anaerobiosis, the "Custer effect". Thus, the fermentative capacity of D. bruxellensis could be further improved by metabolic engineering.

• 出版日期2016-4