Altering n-3 to n-6 polyunsaturated fatty acid ratios affects prostaglandin production by ovine uterine endometrium

作者：Cheng Zhangrui^*; Abayasekara D Robert E; Ward Freya; Preece Daniel M W; Raheem Kabir A; Wathes D Claire

来源：Animal Reproduction Science, 2013, 143(1-4): 38-47.

DOI：10.1016/j.anireprosci.2013.10.015

• Polyunsaturated fatty acid
• Prostaglandins
• Prostaglandin-endoperoxide synthase
• Endometrial cells
• Uterus
• Ewe

Consumption of n-3 polyunsaturated fatty acids (PUFAs) is considered beneficial to health but effects on fertility remain uncertain. This study investigated the effect of n-3 PUFA supplementation on endometrial prostaglandin (PG) production. Ovine uterine endometrial cells were cultured to confluence in DMEM/F12 medium containing 10% foetal bovine serum. Stromal and epithelial cell populations were confirmed by immunocytochemistry. Cultures were supplemented with 0,20 or 100 mu M of alpha-linolenic acid (ALA), stearidonic acid (SDA), eicosapentaenoic acid (EPA) with lipopolysaccharide (LPS) at 0 and 0.11 mu g/ml, or different combinations of EPA with arachidonic acid (AA) in serum-free medium for 24 h. PGs were quantified using radioimmunoassay and PG-endoperoxide synthase (PTGS) isoforms, PGE and PGF synthase (microsomal PGES1 and PGFS) mRNAs by qPCR. LPS increased PGE(2) production significantly without changing PGF(2 alpha), production, causing increased PGE(2):PGF(2 alpha), ratios. ALA and SDA increased PGE(2), PGF(2 alpha), and PGE(2):PGF(2 alpha), ratios (P< 0.05-0.01) while EPA alone did not affect PG generation. AA significantly stimulated PTGS1 and PTGS2 mRNA expression and PGE2 and PGF(2 alpha), production (P< 0.01). The stimulatory effect of AA was attenuated by up to 80% (P< 0.05) when AA was combined with EPA. The PGE(2):PGF(2 alpha), ratio was not affected by AA or EPA alone, but increased when these two PUFAs were combined (P< 0.05). SDA and EPA decreased PTGS1 mRNA expression (P< 0.05) but did not alter PTGS2 expression. EPA and AA up-regulated mPGES1 expression (P< 0.05) without affecting PGFS expression. Since AA is preferentially incorporated in uterine endometrium to produce 2-series PGs, alteration of PG production by EPA may affect many reproductive processes.

• 出版日期2013-12