A Tolerance Gene for Prenylated Flavonoid Encodes a 26S Proteasome Regulatory Subunit in Sophora flavescens

作者：Shitan Nobukazu; Kamimoto Yoshihisa; Minami Shota; Kubo Mizuki; Ito Kozue; Moriyasu Masataka; Yazakj Kazufumi^*

来源：Bioscience Biotechnology and Biochemistry, 2011, 75(5): 982-984.

DOI：10.1271/bbb.100665

• prenylated flavonoids
• Sophora flavescens
• functional screening
• proteasome subunit
• 26S proteasome

Yeast functional screening with a Sophora flavescens cDNA library was performed to identify the genes involved in the tolerant mechanism to the self-producing prenylated flavonoid sophoraflavanone G (SFG). One cDNA, which conferred SFG tolerance, encoded a regulatory particle triple-A ATPase 2 (SfRPT2), a member of the 26S proteasome subunit. The yeast transformant of SfRPT2 showed reduced SFG accumulation in the cells.

• 出版日期2011-5