Adrenal endothelin-1 levels are not associated with aldosterone secretion in primary aldosteronism

作者：Morello F^*; Schiavone D; Mengozzi G; Bertello C; Liew C C; Bi**occi D; Mulatero P; Veglio F

来源：European Journal of Endocrinology, 2009, 160(3): 453-458.

DOI：10.1530/EJE-08-0828

Background: Endothelin-1 (ET-1) may function as an aldosterone secretagogue and. in turn, aldosterone can Upregulate ET-1. expression. Hence, the existence of a feedforward loop involving ETs and aldosterone has been speculated in primary aldosteronism (PA). In the present Study, we sought to examine ET-1, secretion from the adrenal glands in patients with PA.
Design: We determined ET-1 levels in blood samples obtained during adrenal venous sampling of patients affected by PA (n=17). Furthermore, we examined the mRNA expression of the ET system in tissue samples from aldosterone-producing adenomas (APAs, n=9) and control normal adrenals (n = 3).
Methods: Blood ET-1 levels were determined by RIA. Tissue mRNA expression of the ET system was assayed with Affymetrix microarrays.
Results: ET-1. levels did not differ between inferior vena cava and adrenal vein blood in both bilateral adrenal hyperplasia and APA patients. Moreover. cortisol-normalized ET-1 levels did not show lateralized adrenal ET-1 secretion in APAs. Through gene expression profiling with microarray performed in a distinct set of APA individuals (n=9), we confirmed the adrenal expression of a complete ET system, but we did not detect a significant upregulation of ET components within the APA tissue compared with normal adrenals.
Conclusions: The present data argue against the hypothesis of increased ET-1 secretion from APAs and do not support a general role for adrenal ET-1 in the vascular pathophysiology of PA.

• 出版日期2009-3