Amperometric catechol biosensor based on polyaniline-polyphenol oxidase

作者：Tan, Yongyan; Guo, Xiaoxia; Zhang, Jinghui; Kan, Jinqing^*

来源：Biosensors and Bioelectronics, 2010, 25(7): 1681-1687.

DOI：10.1016/j.bios.2009.12.007

• Polyphenol oxidase
• Catechol
• Ionic liquid
• Direct electropolymerization

A novel catechol biosensor was described based on the immobilization of polyphenol oxidase (PPO) into polyaniline (PANI), which was easily constructed by direct electropolymerization of aniline in a solution containing ionic liquid, 1-ethyl-3-methylimidazolium ethyl sulfate (EMIES). The developed biosensor for the detection of catechol has a linear range of 1.25-150 mu mol dm(-3). The maximum response current(I-max) and the Michaelis-Menten constant (k(m)') are 0.62 mu A and 146 mu mol dm(-3), respectively. The activation energy (E-a) of the PPO catalytic reaction is 31.1 kJ mol(-1) in the B-R buffer. The biosensor shows good reproducibility (a relative standard deviation of 3.1% was obtained) and remarkable long-term stability (it retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described.

• 出版日期2010-3-15
• 单位扬州大学